Development of molecular diagnostic assays for seed-borne pathogens of wheat

TitleDevelopment of molecular diagnostic assays for seed-borne pathogens of wheat
Publication TypeConference Paper
Year of Publication2002
AuthorsMcEwan, M., and V. Mulholland
Conference NameProceedings Crop Protection in Northern Britain 2002
Pagination127-132
Conference LocationDundee
Abstract

Seed producers work under severe time constraints during the harvest
period. Decisions on seed treatment during seed production is restricted by the lack of timely results from seed health assays. Currently, advisory testing is carried out using an agar plate test for Microdochium nivale (up to 7 days) and a seed washing/filtration method for Tilletia tritici (24 hours). Although the T. tritici test can be completed within one day, the throughput for this test is low. We describe the development of PCR assays for detection of these pathogens. The PCR Mimic technique (a form of competitive PCR), and real-time PCR were tested for quantification of fungal infection.

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