Antibody Unit

The Antibody Unit was established in 1986 to enable SASA to meet its responsibilities for the health of Scottish seed potatoes and for the quarantine testing of imported potato material. The primary focus of this work has been the development of ELISA assays incorporating monoclonal antibodies (MAbs) for the detection of many indigenous and non-indigenous potato viruses.

Antibodies have been developed for use in testing imported potato breeding material, microplants of existing varieties, leaf samples from field grown plants and tubers. Antibody reagents may also be used in research applications such as Electron Microscopy, membrane blotting and immunocapture techniques for PCR.

Freeze dried conjugated antibodiesMost of the testing reagents we provide are based on MAbs produced in our tissue culture facility. Cell lines producing MAbs have all been developed at SASA and have undergone rigorous evaluation to ensure that the specificity that they exhibit is suitable for the testing systems required. In some cases defined mixtures of MAbs are used to ensure that the isolate range for a product covers the major geographical variants of the virus found in field situations.

*Freeze dried conjugated antibodies are also available on request where long-term storage is required or adverse shipping conditions may potentially affect liquid product.

New products are always under development.

Poster presentations relating to our products

Development of a Quality Assurance System for Plant Virus Testing Reagents  
Plant Virus Testing Reagents
Development of a Monoclonal Antibody for the detection of Potato Mop-Top Virus
SASA's Freezing

Reagent sets for ELISA testing contain two products, a coating antibody which is a purified form of the MAb used to trap virus to the wells of an ELISA plate and a reporter reagent which is the antibody conjugated to the enzyme alkaline phosphatase.

The antibody/enzyme conjugates are made by us using a controlled reaction process which optimises the ratios of antibody and enzyme.

Conjugates produced in this way exhibit high signal to noise ratios in ELISA and are extremely consistant in performance.

Reagents produced by us are tested for specificity and consistency and can be supplied with batch QC data.

BSI/UKASAll of the production steps in the manufacture of our antibodies and conjugates are controlled within a Quality Management system compliant to BSi EN 9001:2015 FM 59966

Current BSi certificate  

Antibody Sales

QUALITY SOLUTIONS TO YOUR TESTING REQUIREMENTS

SASA antibodies and ELISA reagents are produced under a quality system compliant to BS EN ISO 9001 2015. Accreditation to this standard was achieved in July 2001. All SASA antibodies have been extensively tested during development and all ELISA reagent sets can be supplied with Quality Control documentation.

SASA ELISA reagents, previously only available from our Scottish agent can now be purchased directly from us. Our comprehensive list of reagents for European and non-indigenous potato viruses is given below. Evaluation samples are available on request and special rates for bulk purchase may apply.

For all enquiries and to place an order contact:

Craig Douglas
Tel : +44 (0)131 244 8867
Email: Craig.Douglas@sasa.gsi.gov.uk

Orders are normally processed and dispatched on receipt of your request and we can usually arrange for next day delivery if this is required.

UK delivery by post is free of charge.

Courier charges are*

UK delivery £20.00
Europe £40.00
All other countries £50.00

*approximately depending on weights 

PRODUCT INFORMATION

Our reagents are available in 1000 and 5000 unit pack sizes for coating and conjugated antibodies. To simplify ordering our reagents are grouped into our Regular and Specialist lists. 

Antibody Product Brochure
Antibody Evaluation Form
Antibody Request Form
MSDS Antibody Reagents

Regular Products

Potato leafroll virus
Potato virus A
Potato virus M
Potato virus S 
Potato virus V
Potato virus X 
Potato virus Y o, c and n strains
Potato virus Y c strain
Potato virus Y o and c strains
Potato virus Y n strain

Coating antibody 1000 units = £85
Coating antibody 5000 units = £185

Conjugates 1000 units = £125
Conjugates 5000 units = £275

Specialist Products

Arracacha virus B - oca strain
Potato black ringspot virus including Tobacco ringspot virus - Calico strain
Potato latent virus (syn. Red la Soda virus)
Potato mop-top virus 
Potato virus T 
Tomato black ring virus

Coating antibody 1000 units = £125
Coating antibody 5000 units = £385

Conjugates 1000 units = £185
Conjugates 5000 units = £495

We maintain links with other agricultural institutes and many of our antibodies have been validated in a number of locations globally. All of our antibodies for the detection of Andean viruses have been tested at the International Potato Centre (CIP), Lima, Peru.

New products are always under development.

product information

Protocol (ELISA)

DAS ELISA Method

Prepare coating antibody in coating buffer at recommended dilution and dispense (200 μL) into wells of an ELISA plate.

Incubate 4hrs at 37oC.

Wash plate x4 with wash buffer and blot dry.

Prepare sample by homogenising plant material in extraction buffer 1/10 (w/v) and dispense (200 μL) into test wells. Incubate overnight at 4oC.

Wash plate x4 with wash buffer and blot dry.

Dilute antibody-AP conjugate at recommended dilution in extraction buffer and dispense (200 μL) into test wells. Incubate 2hrs 37oC.

Wash plate x4 with wash buffer and blot dry.

Prepare substrate 1mg/mL in substrate buffer and dispense (200 μL) into test wells. Incubate at ambient for 1-2hrs.

Read OD of test wells at 405n.

REAGENTS: Coating buffer, Wash buffer, Extraction buffer, Substrate buffer

NB: The above protocol is used by us for the QC evaluations that are carried out by us on our reagents. Users have found that conditions and reagent volumes may be varied without substantially affecting the results obtained.

Buffer Recipes

DAS-ELISA Method

Prepare coating antibody (MAb) in coating buffer at recommended dilution and dispense (200 µL) into wells of an ELISA plate.

Incubate 4hrs at 37oC.

Wash plate x4 with wash buffer and blot dry.

Prepare sample by homogenising plant material in extraction buffer 1/10 (w/v) and dispense (200 µL) into test wells. Incubate overnight at 4oC.

Wash plate x4 with wash buffer and blot dry.

Dilute antibody-AP conjugate at recommended dilution in extraction buffer and dispense (200 µL) into test wells. Incubate 2hrs 37oC.

Wash plate x4 with wash buffer and blot dry.

Prepare substrate 1mg/mL in substrate buffer and dispense (200 µL) into test wells. Incubate at ambient for 1-2hrs.

Read OD of test wells at 405n.

Buffer Recipes

Coating buffer substance         Amount
Na2CO3                                         0.8g
NaHCO3                                        1.4g
1% NaN3                                       10mL
H2O                                               500mL
pH 9.6

Wash buffer
Phosphate Buffered Saline + 0.05% Tween 20

Extraction buffer substance     Amount
Polyvinyl pyrrolidone                      10g
Bovine serum albumin                    1g
1% NaN3                                        10mL
Wash buffer                                   500mL
pH 7.4

Substrate buffer substance      Amount
Diethanolamine                             48.5mL
1% Sodium azide                           10mL
H2O                                               500mL
pH 9.8

1mg p-nitrophenyl phosphate per mL substate buffer   

Printable version of ELISA Buffer Recipes

SAPS Project

 

Science & Plants for Schools: ELISA kit

 

Science & Plants for Schools (SAPS) supports teachers, technicians and students learning plant science, from primary through to post-16. SAPS have a particular focus on innovative and reliable practical protocols for secondary biology.

SAPS Elisa kit

In 1990 SAPS launched a schools ELISA kit for the detection of Botrytis cinerea and approached the Antibody Unit to see if we would be willing to take over responsibility for the production and supply of the kits directly to schools. We agreed and now supply schools throughout the UK and parts of Europe with this very successful kit. The antibody used in the kit was developed by Molly Dewey of Oxford University and the cell line was gifted by her for use in this project.

Schools and further education institutes order the kits directly from SASA and we also provide a helpdesk facility for any advice that may be required by teachers.

This school and further education ELISA practical kit is a recognised biology practical by organisations including, SQA and AQA and is used by students studying Advanced Higher Biology, A-level biology and equivalent courses. 

ELISA dilution

This ELISA practical kit provides sufficient reagents and equipment for five pairs of students to carry out the experiment. Technical guides and Student guides can be found below.  

The kit is usually available ex stock and costs £35 including postage (ex VAT).

SAPS - PDF files

Student Guide
Technical Guide
Checklist Component

Orders can be placed by:

Phone:   0131 244 8867
Email  :  Craig.Douglas@sasa.gsi.gov.uk
Post   :  Antibody Unit, SASA, Roddinglaw Road, Edinburgh, EH12 9FJ

 

Additional teaching notes and ideas for investigations are available from the Science & Plants for Schools (SAPS) website.